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| Registros recuperados: 16 | |
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| Dourado,Manuella Nóbrega; Andreote,Fernando Dini; Dini-Andreote,Francisco; Conti,Raphael; Araújo,Janete Magali; Araújo,Welington Luiz. |
| The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phylogenetic diversity; Methylotrofics; PPFM; Plant-bacteria interaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100020 |
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| Lacava,Paulo Teixeira; Andreote,Fernando Dini; Araújo,Welington Luiz; Azevedo,João Lúcio. |
| O objetivo deste trabalho foi caracterizar a comunidade bacteriana endofítica de plantas assintomáticas (escapes) e afetadas pela clorose variegada dos citros (CVC) por meio de isolamento em meio de cultura, técnica de gradiente desnaturante em gel de eletroforese (DGGE) e detecção de Methylobacterium mesophilicum e Xyllela fastidiosa por meio de PCR específico, para estudar esta comunidade e sua relação com a ocorrência da CVC. A análise da comunidade bacteriana via DGGE permitiu a detecção de X. fastidiosa, bem como Klebsiella sp. e Acinetobacter sp. como endófitos de citros. Foram observados também Curtobacterium sp., Pseudomonas sp., Enterobacter sp. e Bacillus spp. Utilizando primers específicos, Methylobacterium mesophilicum e X. fastidiosa também... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus sinensis; Xylella fastidiosa; Methylobacterium; Bactérias endofíticas. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000400013 |
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| Rudi Emerson de Lima,Procópio; Araújo,Welington Luiz; Andreote,Fernando Dini; Azevedo,João Lúcio. |
| A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Green fluorescent protein; Bacteria; Host plant; Eucalyptus. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018 |
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| Garcia,William Vilar; Alcântara,Marco Aurélio Kondracki de; Camargo,Otávio Antonio de; Izário Filho,Hélcio José; Andreote,Fernando Dini. |
| Este estudo teve como objetivo avaliar alguns impactos decorrentes do deslocamento miscível de efluente de nitração de uma indústria de explosivos aplicado em colunas de um Latossolo Amarelo, horizonte B (LA-B), submetido aos tratamentos: adição de carbonatos (BASE), ácidos (ACID), fosfato (FOSF), carbonatos e fosfato (BASE-FOSF) e ácidos e fosfatos (ACID-FOSF). A recuperação de nitrogênio em relação ao total aplicado varia entre 10,1 (ACID) e 65,5% (BASE). Há correlação significativa entre as curvas de transposição de N obtidas experimentalmente e as simuladas pelo aplicativo STANMOD para a maioria das colunas (p<0,001). A exceção ocorreu para ACID-FOSF (p=0,202). Não há correlação entre carga eletrostática líquida (CEL) e as variáveis de ajuste do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deslocamento miscível; Nitrogênio; Curvas de transposição; PCR-DGGE. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000100015 |
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| Soares Júnior,Fábio Lino; Dias,Armando Cavalcante Franco; Fasanella,Cristiane Cipola; Taketani,Rodrigo Gouvêa; Lima,André Oliveira de Souza; Melo,Itamar Soares; Andreote,Fernando Dini. |
| The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulose endo-1; 4-β-D-glucanase exo-1; 4-β-D-glucanase salinity. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048 |
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| Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir. |
| A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013 |
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| Sebastianes,Fernanda Luiza de Souza; Maki,Cristina Sayuri; Andreote,Fernando Dini; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida. |
| The Pink Disease is caused by Erythricium salmonicolor, which attacks broad hosts, such as citrus, coffee, rubber, Eucalyptus spp. and Acacia spp., infecting mainly branches. This disease became a serious problem in Brazil, reducing the citrus production up to 10%. However the genetic diversity and compatibility of the fungus E. salmonicolor from Brazilian citrus plants is not yet evaluated. Therefore, the aims of this study were to evaluate: i) the genetic variability of E. salmonicolor in the São Paulo and Minas Gerais States by the RAPD technique, and ii) the vegetative compatibility between these isolates. After RAPD analysis, six distinct groups were observed without correlation between the isolation site or host species. In the vegetative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corticium salmonicolor; RAPD; Pink Disease; Hyphal fusion; Heterokariosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200009 |
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| Dias,Armando Cavalcante Franco; Taketani,Rodrigo Gouveia; Andreote,Fernando Dini; Luvizotto,Danice Mazzer; Silva,João Luis da; Nascimento,Rosely dos Santos; Melo,Itamar Soares de. |
| Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species (Avicenia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture-independent profiling; Plant genotype; Surface leaves. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200030 |
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| Dourado,Manuella Nóbrega; Bogas,Andrea Cristina; Pomini,Armando M.; Andreote,Fernando Dini; Quecine,Maria Carolina; Marsaioli,Anita J.; Araújo,Welington Luiz. |
| Bacteria from the genus Methylobacterium interact symbiotically (endophytically and epiphytically) with different plant species. These interactions can promote plant growth or induce systemic resistance, increasing plant fitness. The plant colonization is guided by molecular communication between bacteria-bacteria and bacteria-plants, where the bacteria recognize specific exuded compounds by other bacteria (e.g. homoserine molecules) and/or by the plant roots (e.g. flavonoids, ethanol and methanol), respectively. In this context, the aim of this study was to evaluate the effect of quorum sensing molecules (N-acyl-homoserine lactones) and plant exudates (including ethanol) in the expression of a series of bacterial genes involved in Methylobacterium-plant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endophyte; Methylobacterium; Quantitative PCR (qPCR); Plant-bacteria Interaction; Homoserine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400044 |
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| Rodrigues,Maria Beatriz Calderan; Andreote,Fernando Dini; Spósito,Marcel Bellato; Aguillar-Vildoso,Carlos Ivan; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida. |
| O objetivo deste trabalho foi avaliar a resistência de Guignardia citricarpa aos fungicidas carbendazim e piraclostrobina, por meio de avaliação do crescimento em meio de cultura, decomposição de tecido foliar e produção de corpos de frutificação. Para isso, o fungo G. citricarpa foi isolado de lesões de frutos de laranja (Citrus sinensis), produzidos em área com intensa aplicação de fungicida. Os isolados obtidos foram avaliados quanto à sensibilidade aos fungicidas piraclostrobina e carbendazim, nas dosagens de 0,5, 1 e 2 µg mL-1 de i.a., para se verificar o efeito da pressão de seleção causada pelo uso destes compostos em áreas citrícolas. Embora tenha sido observada redução efetiva no número de estruturas reprodutivas e na decomposição de folhas e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mancha-preta dos citros; Pinta-preta; Tolerância a fungicidas; Citricultura. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300004 |
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| Rigonato,Janaina; Kent,Angela D.; Gumiere,Thiago; Branco,Luiz Henrique Zanini; Andreote,Fernando Dini; Fiore,Marli Fátima. |
| ABSTRACT Variations in microbial communities promoted by alterations in environmental conditions are reflected in similarities/differences both at taxonomic and functional levels. Here we used a natural gradient within mangroves from seashore to upland, to contrast the natural variability in bacteria, cyanobacteria and diazotroph assemblages in a pristine area compared to an oil polluted area along a timespan of three years, based on ARISA (bacteria and cyanobacteria) and nifH T-RFLP (diazotrophs) fingerprinting. The data presented herein indicated that changes in all the communities evaluated were mainly driven by the temporal effect in the contaminated area, while local effects were dominant on the pristine mangrove. A positive correlation of community... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mangrove; Cyanobacteria; Diazotrophs; Oil contamination; NifH T-RFLP; ARISA. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100087 |
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| Registros recuperados: 16 | |
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